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Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii

Title data

Xu, Nannan ; Oltmanns, Anne ; Zhao, Longsheng ; Girot, Antoine ; Karimi, Marzieh ; Hoepfner, Lara ; Kelterborn, Simon ; Scholz, Martin ; Beißel, Julia ; Hegemann, Peter ; Bäumchen, Oliver ; Liu, Lu-Ning ; Huang, Kaiyao ; Hippler, Michael:
Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii.
In: eLIFE. Vol. 9 (10 December 2020) . - No. e58805.
ISSN 2050-084X
DOI: https://doi.org/10.7554/eLife.58805

Abstract in another language

For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion. However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes. To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions. Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces. This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces. Notably, assembly, intraflagellar transport, and protein import into flagella are not affected by altered N-glycosylation. Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics V
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics V > Chair Experimental Physics V - Univ.-Prof. Dr. Oliver Bäumchen
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 530 Physics
500 Science > 570 Life sciences, biology
Date Deposited: 11 Jan 2021 12:59
Last Modified: 11 Jan 2021 12:59
URI: https://eref.uni-bayreuth.de/id/eprint/61548