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Resolution in optical microscopy

Title data

Jonkman, James E. N. ; Swoger, Jim ; Kress, Holger ; Rohrbach, Alexander ; Stelzer, Ernst H. K.:
Resolution in optical microscopy.
In: Marriott, Gerard ; Parker, Ian (ed.): Biophotonics. Part A. - Amsterdam : Academic Press , 2003 . - pp. 416-446 . - (Methods in Enzymology ; 360 )
ISBN 0-12-182263-X
DOI: https://doi.org/10.1016/S0076-6879(03)60122-9

Official URL: Volltext

Abstract in another language

Publisher Summary This chapter describes resolution in optical microscopy. Optical microscopes are fundamentally limited in the resolution they can achieve. The resolution depends on the wavelength of the light (both incident and detected), on the numerical aperture (NA) of the optical arrangement, and on the specimen to be observed or the experiment to be performed. Live specimens are also dynamic and sensitive to photobleaching and thermal damage, which imposes a limit on the duration for which they can be observed and on the power of the incident light. Fluorescence is excited throughout its illumination cone, but only fluorescence emitted from the focal point is imaged through the confocal pinhole to the detector. A useful tool for comparing the performance of optical microscopes is the point-spread function (PSF). The PSF can be defined in two complementary. The intensity PSF (hereafter referred to simply as the PSF) can be measured by taking images of—for example, a subresolution bead as it is scanned through the focus of a microscope. Real specimens are, of course, rarely point sources.

Further data

Item Type: Article in a book
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Professor Experimental Physics VI - Biologial Physics > Professor Experimental Physics VI - Biologial Physics - Univ.-Prof. Dr. Holger Kreß
Result of work at the UBT: No
DDC Subjects: 500 Science > 530 Physics
500 Science > 570 Life sciences, biology
Date Deposited: 26 Feb 2021 08:37
Last Modified: 26 Feb 2021 08:38
URI: https://eref.uni-bayreuth.de/id/eprint/63424