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Probing the Cell Membrane by Magnetic Particle Actuation and Euler Angle Tracking

Titelangaben

Irmscher, Matthias ; de Jong, Arthur M. ; Kress, Holger ; Prins, Menno W. J.:
Probing the Cell Membrane by Magnetic Particle Actuation and Euler Angle Tracking.
In: Biophysical Journal. Bd. 102 (2012) Heft 3 . - S. 698-708.
ISSN 1542-0086
DOI: https://doi.org/10.1016/j.bpj.2011.12.054

Volltext

Link zum Volltext (externe URL): Volltext

Abstract

The mechanical properties of the cell membrane and the subjacent actin cortex are determinants of a variety of processes in immunity and cell division. The lipid bilayer itself and its connection to the actin cortex are anisotropic. An accurate description of the mechanical structure of the cell membrane and the involved dynamics therefore necessitates a measurement technique that can capture the inherent anisotropy of the system. Here, we combine magnetic particle actuation with rotational and translational particle tracking to simultaneously measure the mechanical stiffness of monocytic cells in three rotational and two translational directions. When using particles that bind via integrins to the cell membrane and the subjacent cortex, we measured an isotropic stiffness and a characteristic power-law dependence of the shear modulus on the applied frequency. When using particles functionalized with immunoglobulin G, we measured an anisotropic stiffness with a 10-fold-reduced value in one dimension. We suggest that the observed reduced stiffness in the plane of the cell membrane is caused by a local detachment of the lipid bilayer from the subjacent cytoskeletal cortex. We expect that our technique will enable new insights into the mechanical properties of the cell membrane that will help us to better understand membrane processes such as phagocytosis and blebbing.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Zusätzliche Informationen: H. Kress and M. Prins are joint corresponding authors
Institutionen der Universität: Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik > Professur Experimentalphysik VI - Biologische Physik - Univ.-Prof. Dr. Holger Kreß
Fakultäten
Fakultäten > Fakultät für Mathematik, Physik und Informatik
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 26 Feb 2021 09:39
Letzte Änderung: 25 Apr 2022 13:12
URI: https://eref.uni-bayreuth.de/id/eprint/63452