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A method for time-resolved measurements of the mechanics of phagocytic cups

Titelangaben

Irmscher, Matthias ; de Jong, Arthur M. ; Kress, Holger ; Prins, Menno W. J.:
A method for time-resolved measurements of the mechanics of phagocytic cups.
In: Interface : Journal of the Royal Society. Bd. 10 (2013) Heft 82 . - 20121048.
ISSN 1742-5662
DOI: https://doi.org/10.1098/rsif.2012.1048

Abstract

The internalization of matter by phagocytosis is of key importance in the defence against bacterial pathogens and in the control of cancerous tumour growth. Despite the fact that phagocytosis is an inherently mechanical process, little is known about the forces and energies that a cell requires for internalization. Here, we use functionalized magnetic particles as phagocytic targets and track their motion while actuating them in an oscillating magnetic field, in order to measure the translational and rotational stiffnesses of the phagocytic cup as a function of time. The measured evolution of stiffness reveals a characteristic pattern with a pronounced peak preceding the finalization of uptake. The measured stiffness values and their time dependence can be interpreted with a model that describes the phagocytic cup as a prestressed membrane connected to an elastically deformable actin cortex. In the context of this model, the stiffness peak is a direct manifestation of a previously described mechanical bottleneck, and a comparison of model and data suggests that the membrane advances around the particle at a speed of about 20 nm s−1. This approach is a novel way of measuring the progression of emerging phagocytic cups and their mechanical properties in situ and in real time.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Zusätzliche Informationen: H. Kress and M. Prins are joint corresponding authors
Institutionen der Universität: Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik > Professur Experimentalphysik VI - Biologische Physik - Univ.-Prof. Dr. Holger Kreß
Fakultäten
Fakultäten > Fakultät für Mathematik, Physik und Informatik
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 26 Feb 2021 08:05
Letzte Änderung: 17 Aug 2023 11:55
URI: https://eref.uni-bayreuth.de/id/eprint/63453