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Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

Title data

Yandrapalli, Naresh ; Petit, Julien ; Bäumchen, Oliver ; Robinson, Tom:
Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics.
In: Communications Chemistry. Vol. 4 (29 June 2021) Issue 1 .
ISSN 2399-3669
DOI: https://doi.org/10.1038/s42004-021-00530-1

Official URL: Volltext

Abstract in another language

Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on the addition of additives such as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 µm with either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality, and stability of the membranes are validated by lipid diffusion, protein incorporation, and leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e., by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This robust method capable of creating truly biomimetic artificial cells in high-throughput will prove valuable for bottom-up synthetic biology and the understanding of membrane function.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics V > Chair Experimental Physics V - Univ.-Prof. Dr. Oliver Bäumchen
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 01 Jul 2021 07:32
Last Modified: 01 Jul 2021 07:32
URI: https://eref.uni-bayreuth.de/id/eprint/66390