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Probing local chromatin dynamics by tracking telomeres

Title data

Benelli, Rebecca ; Weiss, Matthias:
Probing local chromatin dynamics by tracking telomeres.
In: Biophysical Journal. Vol. 121 (19 July 2022) Issue 14 . - pp. 2684-2692.
ISSN 1542-0086
DOI: https://doi.org/10.1016/j.bpj.2022.06.020

Abstract in another language

Chromatin dynamics is key for cell viability and replication. In interphase, chromatin is decondensed, allowing the transcription machinery to access a plethora of DNA loci. Yet, decondensed chromatin occupies almost the entire nucleus, suggesting that DNA molecules can hardly move. Recent reports have even indicated that interphase chromatin behaves like a solid body on mesoscopic scales. To explore the local chromatin dynamics, we have performed single-particle tracking on telomeres under varying conditions. We find that mobile telomeres feature, under all conditions, a strongly subdiffusive, antipersistent motion that is consistent with the monomer motion of a Rouse polymer in viscoelastic media. In addition, telomere trajectories show intermittent accumulations in local niches at physiological conditions, suggesting that the surrounding chromatin reorganizes on these timescales. Reducing the temperature or exposing cells to osmotic stress resulted in a significant reduction of mobile telomeres and the number of visited niches. Altogether, our data indicate a vivid local chromatin dynamics, akin to a semidilute polymer solution, unless perturbations enforce a more rigid or entangled state of chromatin.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter > Chair Experimental Physics I - Physics of Living Matter - Univ.-Prof. Dr. Matthias Weiss
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 05 Aug 2022 07:12
Last Modified: 23 Aug 2022 13:23
URI: https://eref.uni-bayreuth.de/id/eprint/71347