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RGD-functionalisation of PLLA nanofibers by surface coupling using plasma treatment : influence on stem cell differentiation

Title data

Paletta, Jürgen R. J. ; Bockelmann, Sarah ; Walz, Andreas ; Theisen, Christina ; Wendorff, Joachim H. ; Greiner, Andreas ; Fuchs-Winkelmann, Susanne:
RGD-functionalisation of PLLA nanofibers by surface coupling using plasma treatment : influence on stem cell differentiation.
In: Journal of Materials Science: Materials in Medicine. Vol. 21 (April 2010) Issue 4 . - pp. 1363-1369.
ISSN 0957-4530
DOI: https://doi.org/10.1007/s10856-009-3947-2

Official URL: Volltext

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The aim of this study was to functionalize the surface of synthetic poly-(l-lactic) (PLLA) nanofibers with RGD peptide, in order to promote growth and osteogenic differentiation of human mesenchymal stem cells (hMSC) in vitro. The cRGD was coupled onto PLLA nanofibers using oxygen plasma combined with EDC/sulfo-NHS activation. Matrices were seeded with hMSC and cultivated over a period of 22 days under growth conditions and analyzed during the course of cultivation. The plasma activation of PLLA nanofibers resulted in a reduction of hydrophobicity as well as a formation of carboxyl groups on the surface of the fibers. Furthermore, maximum load, but not young's modulus was influenced by the treatment with oxygen plasma. When hMSC were cultured onto the cRGD functionalized scaffolds, cells showed no increased proliferation or cell density but an induction of genes associated with the osteoblast lineage. In brief, this study indicates that functional peptides of the extracellular matrix can be coupled onto PLLA nanofibers using plasma treatment in combination with EDC/sulfo-NHS treatment. These groups are accessible for the growing cell and mediate probably some osteoinductive properties of collagen nanofibers.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: MARROW STROMAL CELLS; SMOOTH-MUSCLE-CELLS; IN-VITRO; OSTEOGENIC DIFFERENTIATION; GENE-EXPRESSION; MECHANICAL-PROPERTIES; COLLAGEN NANOFIBERS; BONE-FORMATION; SCAFFOLDS; ADHESION
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 01 Apr 2015 09:51
Last Modified: 16 Apr 2015 13:42
URI: https://eref.uni-bayreuth.de/id/eprint/9559