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Effect of direct RGD incorporation in PLLA nanofibers on growth and osteogenic differentiation of human mesenchymal stem cells

Title data

Schofer, Markus D. ; Boudriot, Ulrich ; Bockelmann, Sarah ; Walz, Andreas ; Wendorff, Joachim H. ; Greiner, Andreas ; Paletta, Jürgen R. J. ; Fuchs-Winkelmann, Susanne:
Effect of direct RGD incorporation in PLLA nanofibers on growth and osteogenic differentiation of human mesenchymal stem cells.
In: Journal of Materials Science: Materials in Medicine. Vol. 20 (July 2009) Issue 7 . - pp. 1535-1540.
ISSN 0957-4530
DOI: https://doi.org/10.1007/s10856-009-3719-z

Official URL: Volltext

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The aim of this study was to functionalize synthetic poly-(L-lactic) (PLLA) nanofibers by direct incorporation of cRGD, in order to promote adhesion, growth and osteogenic differentiation of human mesenchymal stem cells (hMSC) in vitro. The cRGD was incorporated into PLLA nanofibers either by emulsion [PLLA-cRGD (d)] or suspension [PLLA-cRGD (s)]. Matrices were seeded with hMSC and cultivated over a period of 28 days under growth conditions and analyzed during the course. Although the mode of incorporation resulted in different distributions of the RGD peptide, it had no impact on the fiber characteristics when compared to corresponding unblended PLLA control fibers. However, hMSC showed better adherence on PLLA-cRGD (d). Nevertheless, this advantage was not reflected during the course of cultivation. Furthermore, the PLLA-cRGD (s) fibers mediated the osteogenic potential of collagen (determined as the expression and deposition of collagen and osteocalcin) to some extent. Further studies are needed in order to optimize the RGD distribution and concentration.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: GENE-EXPRESSION; COLLAGEN NANOFIBERS; CONTROLLED-RELEASE; I COLLAGEN; ELECTROSPUN; SCAFFOLDS; FIBERS; ADHESION; ACID); BIOMATERIALS
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 02 Apr 2015 14:02
Last Modified: 02 Apr 2015 14:02
URI: https://eref.uni-bayreuth.de/id/eprint/9598