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Influence of nanofibers on the growth and osteogenic differentiation of stem cells: a comparison of biological collagen nanofibers and synthetic PLLA fibers

Title data

Schofer, Markus D. ; Boudriot, Ulrich ; Wack, Christina ; Leifeld, Irini ; Gräbedünkel, Christian ; Dersch, Roland ; Rudisile, Markus ; Wendorff, Joachim H. ; Greiner, Andreas ; Paletta, Jürgen R. J. ; Fuchs-Winkelmann, Susanne:
Influence of nanofibers on the growth and osteogenic differentiation of stem cells: a comparison of biological collagen nanofibers and synthetic PLLA fibers.
In: Journal of Materials Science: Materials in Medicine. Vol. 20 (March 2009) Issue 3 . - pp. 767-774.
ISSN 0957-4530
DOI: https://doi.org/10.1007/s10856-008-3634-8

Official URL: Volltext

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The aim of this study was to compare biological collagen I (ColI) and synthetic poly-(l-lactide) (PLLA) nanofibers concerning their stability and ability to promote growth and osteogenic differentiation of human mesenchymal stem cells in vitro. Matrices were seeded with human stem cells and cultivated over a period of 28 days under growth and osteoinductive conditions and analyzed during the course. During this time the PLLA nanofibers remained stable while the presence of cells resulted in an attenuation of the ColI nanofiber mesh. Although there was a tendency for better growth and osteoprotegerin production of stem cells when cultured on collagen nanofibers, there was no significant difference compared to PLLA nanofibers or controls. The gene expression of alkaline phosphate, osteocalcin and collagen I diminished in the initial phase of cultivation independent of the polymer used. In the case of PLLA fibers, this gene expression normalized during the course of cultivation, whereas the presence of collagen nanofibers resulted in an increased gene expression of osteocalcin and collagen during the course of the experiment. Taken together the PLLA fibers were easier to produce, more stable and did not compromise growth and differentiation of stem cells over the course of experiment. On the other hand, collagen nanofibers supported the differentiation process to some extent. Nevertheless, the need for fixation as well as the missing stability during cell culture requires further work.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: PEPTIDE-AMPHIPHILE NANOFIBERS; IN-VITRO; GENE-EXPRESSION; SCAFFOLD; CULTURE; POLYCAPROLACTONE; FIBROBLASTS; CARTILAGE
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 02 Apr 2015 14:08
Last Modified: 02 Apr 2015 14:08
URI: https://eref.uni-bayreuth.de/id/eprint/9603