Title data
Kok, Peter ; Köhler, Jürgen ; Groenen, Edgar J. J. ; Gebhard, Ronald ; van der Hoef, Ineke ; Lugtenburg, Johan ; Farhoosh, Roya ; Frank, Harry A.:
Resonance Raman spectroscopy of 2H-labelled spheroidenes in petroleum ether and in the Rhodobacter sphaeroides reaction centre.
In: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.
Vol. 53
(1997)
Issue 3
.
- pp. 381-392.
ISSN 1873-3557
DOI: https://doi.org/10.1016/S1386-1425(96)01845-8
Abstract in another language
As a step towards the structural analysis of the carotenoid spheroidene in the Rhodobacter sphaeroides reaction centre, we present the resonance Raman spectra of 14-2H, 15-2H, 15'-2H, 14'-2, 14,15'-2H2, and 15,15'-2H2 spheroidenes in petroleum ether and, except for 14,15'-2H2 spheroidene, in the Rb. sphaeroides R26 reaction center (RC). Analysis of the spectral changes upon isotopic substitution allows a qualitative assignment of most of the vibrational bands to be made. For the all-trans spheroidenes in solution the resonance enhancement of the Raman bands is determined by the participation of carbon-carbon stretching modes in the centre of the conjugated chain, the C9 to C15' region. For the RC-bound 15,15'-cis spheroidenes, enhancement is determined by the participation of carbon-carbon stretching modes in the centre of the molecule, the C13 to C13' region. Comparison of the spectra in solution and in the RC reveals evidence for an out-of-plane distortion of the RC-bound spheroidene in the central C14 to C14' region of the carotenoid. The characteristic 1240 cm-1 band in the spectrum of the RC-bound spheroidene has been assigned to a normal mode that contains the coupled C12-C13 and C13'-C12' stretch vibrations.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Keywords: | Rhodobacter Sphaeroides |
Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter > Chair Experimental Physics IX - Spectroscopy of Soft Matter - Univ.-Prof. Dr. Jürgen Köhler Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter |
Result of work at the UBT: | No |
DDC Subjects: | 500 Science > 530 Physics |
Date Deposited: | 11 Apr 2018 10:28 |
Last Modified: | 05 Aug 2023 02:49 |
URI: | https://eref.uni-bayreuth.de/id/eprint/43354 |