Title data
Krapf, Lisa ; Dezi, Manuela ; Reichstein, Werner ; Köhler, Jürgen ; Oellerich, Silke:
AFM characterization of spin-coated multilayered dry lipid films prepared from aqueous vesicle suspensions.
In: Colloids and Surfaces B: Biointerfaces.
Vol. 82
(2011)
Issue 1
.
- pp. 25-32.
ISSN 1873-4367
DOI: https://doi.org/10.1016/j.colsurfb.2010.08.006
Abstract in another language
We present a detailed AFM study on multilayered dry lipid films prepared from aqueous vesicle suspensions. Different preparation techniques were applied in order to optimize the preparation of homogeneous lipid films of various film thicknesses. Suspensions of preformed DOPC/DPPC vesicles were adsorbed onto indium tin oxide-coated glass coverslips, a substrate also commonly employed for the formation of giant liposomes. We found that the homogeneity of the lipid films could substantially be improved when applying a spin-coating step during the film preparation. These films were much more homogeneous than those prepared by conventional drop-casting and in addition the film thickness could be controlled. When using a combination of vesicle adsorption and spin-coating the quality and thickness of the films depended crucially on the lipid concentration of the vesicle suspension, the adsorption temperature and the adsorption time. For lipid films prepared by direct spin-coating the lipid concentration and the applied spin-coating sequence were critical parameters for the quality and thickness of the deposited lipid films.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Keywords: | Spin-Coating |
Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter > Chair Experimental Physics IX - Spectroscopy of Soft Matter - Univ.-Prof. Dr. Jürgen Köhler Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 530 Physics |
Date Deposited: | 11 Apr 2018 06:57 |
Last Modified: | 09 Jun 2023 08:17 |
URI: | https://eref.uni-bayreuth.de/id/eprint/43356 |