Title data
Lang, Erwin ; Baier, Jürgen ; Köhler, Jürgen:
Epifluorescence, confocal and total internal reflection microscopy for single-molecule experiments : a quantitative comparison.
In: Journal of Microscopy.
Vol. 222
(2006)
Issue 2
.
- pp. 118-123.
ISSN 1365-2818
DOI: https://doi.org/10.1111/j.1365-2818.2006.01579.x
Abstract in another language
Epifluorescence, confocal and total internal reflection microscopy are the most widely used techniques for optical single-molecule experiments. Employing these methods, we recorded the emission intensity of the same single molecule as a function of the excitation rate under otherwise identical experimental conditions. Evaluation of these data provides a quantitative comparison of the signal-to-background ratios that can be achieved for the three microscopic techniques.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Keywords: | TIR microscopy |
Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter > Chair Experimental Physics IX - Spectroscopy of Soft Matter - Univ.-Prof. Dr. Jürgen Köhler Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 530 Physics |
Date Deposited: | 12 Apr 2018 11:45 |
Last Modified: | 12 Apr 2018 11:45 |
URI: | https://eref.uni-bayreuth.de/id/eprint/43361 |