at Google ScholarTitle data
van Oijen, Antoine M. ; Köhler, Jürgen ; Schmidt, Jan ; Müller, M. ; Brakenhoff, G. J.:
Far-field fluorescence microscopy beyond the diffraction limit.
In: Journal of the Optical Society of America A.
Vol. 16
(1999)
Issue 4
.
- pp. 909-915.
ISSN 1520-8532
DOI: https://doi.org/10.1364/JOSAA.16.000909
Abstract in another language
A technique has been developed to obtain three-dimensional structural information on a length scale well below the Rayleigh length with conventional far-field optics. By spectrally selecting a single molecule with high-resolution laser spectroscopy and using a CCD camera to register the spatial distribution of the emitted photons in three dimensions, one can determine the position of a molecule with unprecedented accuracy. One can resolve details in the specimen with sub-diffraction-limited resolution in three dimensions by applying this procedure to as many molecules as are present in the same diffraction-limited volume and obtaining their mutual positions. The feasibility of this technique is demonstrated for the system of pentacene in p-terphenyl at cryogenic temperatures for which molecules were localized with an accuracy of better than 40 nm in the lateral and 100 nm in the axial directions.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | organic compounds |
| Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter > Chair Experimental Physics IX - Spectroscopy of Soft Matter - Univ.-Prof. Dr. Jürgen Köhler Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics IX - Spectroscopy of Soft Matter |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 530 Physics |
| Date Deposited: | 11 Apr 2018 11:30 |
| Last Modified: | 16 Apr 2018 09:40 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/43415 |