Identification of valine/leucine/isoleucine and threonine/alanine/glycine proton-spin systems of Escherichia coli adenylate kinase by selective deuteration and selective protonation

Title data

Bock-Möbius, Imke ; Brune, Martin ; Wittinghofer, Alfred ; Zimmermann, Herbert ; Leberman, Reuben ; Dauvergne, Marie-Thérèse ; Zimmermann, Sabine ; Brandmeier, Birgit ; Rösch, Paul:
Identification of valine/leucine/isoleucine and threonine/alanine/glycine proton-spin systems of Escherichia coli adenylate kinase by selective deuteration and selective protonation.
In: Biochemical Journal. Vol. 273 (1991) Issue 2 . - pp. 311-316.
ISSN 1470-8728
DOI: https://doi.org/10.1042/bj2730311

Official URL:

Abstract in another language

Adenylate kinase from two types of Escherichia coli strains, a wild-type and a leucine-auxotrophic strain, was purified. On the one hand, growing the leucine-auxotrophic bacteria on a medium containing deuterated leucine yielded E. coli adenylate kinase with all leucine residues deuterated. On the other hand, by growing the wild-type bacteria on deuterated medium with phenylalanine, threonine and isoleucine present as protonated specimens, 80% randomly deuterated enzyme with protonated phenylalanine, threonine and isoleucine residues could be prepared. Use of these proteins enabled identification of the spin systems of these amino acid residues in the n.m.r. spectra of the protein.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry
Result of work at the UBT:	No
DDC Subjects:	500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology
Date Deposited:	09 Jan 2019 08:08
Last Modified:	31 Mar 2022 13:09
URI:	https://eref.uni-bayreuth.de/id/eprint/46834