at Google ScholarTitle data
Faber, Cornelius ; Lindemann, Almut ; Sticht, Heinrich ; Ejchart, Andrzej ; Kungl, Andreas ; Susani, Markus ; Frank, Rainer W. ; Kraft, Dietrich ; Breitenbach, Michael ; Rösch, Paul:
Secondary Structure and Tertiary Fold of the Birch Pollen Allergen Bet v 1 in Solution.
In: The Journal of Biological Chemistry.
Vol. 271
(1996)
Issue 32
.
- pp. 19243-19250.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.271.32.19243
Abstract in another language
Bet v 1 is the major birch pollen allergen and therefore the main cause of type I allergies observed in early spring. It is composed of 159 amino acid residues adding up to a molecular mass of 17 kDa. We determined the secondary structure and tertiary fold of full-length Bet v 1 by NMR spectroscopy. Two- and three-dimensional NMR measurements suggest that Bet v 1 is a globular monomer in solution with a high content of well defined secondary structure. Of the total of 159 residues, 135 could be sequentially assigned, using an improved assignment strategy based mainly on heteronuclear experiments. An improved strategy for structure calculation revealed three helices and two beta-sheets as major elements of secondary structure. The globular tertiary structure is mainly stabilized by two antiparallel beta-sheets. The two helices at the C terminus are in accordance with the results from the solution structure of the chemically synthesized peptide Bet v 1-(125-154). This peptide is composed of two helices connected by a hinge. The structural features of Bet v 1 are highly similar to those found in the Ambrosia allergen Amb t V.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 11 Jan 2019 08:05 |
| Last Modified: | 31 Mar 2022 13:33 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/46883 |