Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory- activity protein

Title data

Sticht, Heinrich ; Auer, Manfred ; Schmitt, Bettina ; Besemer, Jürgen ; Horcher, Markus ; Kirsch, Thomas ; Lindley, Ivan J. D. ; Rösch, Paul:
Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory- activity protein.
In: European Journal of Biochemistry. Vol. 235 (1996) Issue 1-2 . - pp. 26-35.
ISSN 1432-1033
DOI: https://doi.org/10.1111/j.1432-1033.1996.00026.x

Official URL:

Abstract in another language

A 72-amino-acid chimeric protein, Chi1, was constructed from the N-terminal part of interleukin 8, IL-8-(1-53), and the C-terminal part of melanoma growth stimulatory activity, MGSA-(54-72). Chi1 protein showed receptor-binding specificity and biological activity similar, but not identical to IL-8 and decidedly different from MGSA. The structure of Chi1 was determined in solution by two-dimensional NMR and molecular-dynamics calculations. The structure resembled the structures of MGSA and IL-8 closely, containing a triple-stranded beta-sheet in the IL-8 part and an amphipathic alpha-helix in the MGSA part. Chi1 formed dimers at millimolar concentrations via the first strand from the N-terminus, analogous to IL-8 and MGSA. In contrast to the latter molecules, however, the alpha-helix of Chi1 did not pack against the beta-sheet part, but was an independent structural element. This structural difference could be explained mainly by the modulation of hydrophobic interactions between the helix and the rest of the protein in Chi1 as compared to IL-8 and MGSA. It is concluded that tight helix packing is not required for receptor binding and biological activity of Chi1.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry
Result of work at the UBT:	Yes
DDC Subjects:	500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology
Date Deposited:	11 Jan 2019 08:29
Last Modified:	31 Mar 2022 13:28
URI:	https://eref.uni-bayreuth.de/id/eprint/46886