at Google ScholarTitle data
Allen, Thomas E. ; Heidmann, Stefan ; Reed, RoseMary ; Myler, Peter J. ; Göringer, H. Ulrich ; Stuart, Kenneth:
Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.
In: Molecular and Cellular Biology.
Vol. 18
(1998)
Issue 10
.
- pp. 6014-6022.
ISSN 1098-5549
DOI: https://doi.org/10.1128/mcb.18.10.6014
Abstract in another language
RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitachondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of preciously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA, These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | Mitochondrial Ribonucleoprotein Complexes; Precursor Messenger-RNA; Kinetoplastid Mitochondria; In-Vitro; Molecules; Grna; Information; Transcript; Mechanism; Model |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 10 Mar 2020 12:36 |
| Last Modified: | 09 Jun 2022 07:54 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54552 |