at Google ScholarTitle data
Wang, Bingbing ; Salavati, Reza ; Heidmann, Stefan ; Stuart, Kenneth:
A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing.
In: RNA : a Publication of the RNA Society.
Vol. 8
(2002)
Issue 4
.
- pp. 548-554.
ISSN 1469-9001
DOI: https://doi.org/10.1017/s135583820202962x
Abstract in another language
Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by Incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing substrates that are modeled on hammerhead ribozymes were designed with catalytic cores that contained or lacked additional uridylates (Us). They proved to be sensitive reporters of editing activity when used for in vitro assays. A deletion editing substrate that is based on A6 pre-mRNA had no ribozyme activity, but its incubation with gRNA and mt extract resulted In its deletion editing and production of a catalytically active ribozyme. Hammerhead ribozymes are thus sensitive tools to assay in vitro RNA editing.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | gRNA; Trypanosoma brucei; uridylate deletion; uridylate insertion; Precursor Messenger-RNA; Trypaosoma-Brucei; Guide RNAS; In-Vitro; Cleavage; GRNA; Mitochondria; Mechanism; Specificity; Efficiency |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Genetics Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 10 Mar 2020 13:07 |
| Last Modified: | 26 Oct 2022 12:33 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54598 |