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Mitochondria-targeted green fluorescent proteins : convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae

Title data

Westermann, Benedikt ; Neupert, Walter:
Mitochondria-targeted green fluorescent proteins : convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae.
In: Yeast. Vol. 16 (November 2000) Issue 15 . - pp. 1421-1427.
ISSN 1097-0061
DOI: https://doi.org/10.1002/1097-0061(200011)16:15<1421::AID-YEA624>3.0.CO;2-U

Abstract in another language

We describe the construction and characterization of a novel set of plasmids for expression of mitochondria-targeted green fluorescent protein (GFP) in Saccharomyces cerevisiae. The vectors include constructs with strong regulatable and constitutive promoters, four different auxotrophic markers for yeast transformation, and a green (S65T) and a blue-shifted (P4-3) variant of GFP. Mitochondria are brightly fluorescent in living yeast cells grown on different carbon sources and at different temperatures, with virtually no background staining. Specific staining of mitochondria is also shown for a respiratory-deficient mutant with abnormal mitochondrial morphology. The plasmids facilitate convenient analysis of mutants defective in mitochondrial morphology or inheritance and, at the same time, are suitable vectors for easy construction of different kinds of GFP fusion proteins to study various aspects of organelle biogenesis in living yeast cells.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology > Professor Cell Biology - Univ.-Prof. Dr. Benedikt Westermann
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 15 Oct 2020 07:22
Last Modified: 15 Oct 2020 07:22
URI: https://eref.uni-bayreuth.de/id/eprint/58356