Integrin-Induced PIP5K1C Kinase Polarization Regulates Neutrophil Polarization, Directionality, and In Vivo Infiltration

Title data

Xu, Wenwen ; Wang, Ping ; Petri, Björn ; Zhang, Yong ; Tang, Wenwen ; Sun, Le ; Kress, Holger ; Manes, Thomas D. ; Shi, Yan ; Kubes, Paul ; Wu, Dianqing:
Integrin-Induced PIP5K1C Kinase Polarization Regulates Neutrophil Polarization, Directionality, and In Vivo Infiltration.
In: Immunity. Vol. 33 (2010) Issue 3 . - pp. 340-350.
ISSN 1074-7613
DOI: https://doi.org/10.1016/j.immuni.2010.08.015

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Abstract in another language

Summary Neutrophils are important in innate immunity and acute inflammatory responses. However, the regulation of their recruitment to sites of inflammation has not been well characterized. Here, we investigated the kinase PIP5K1C and showed that PIP5K1C deficiency impaired neutrophil recruitment because of an adhesion defect. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants. Integrins could induce polarization of an isoform of PIP5K1C, PIP5K1C-90, in neutrophils through intracellular vesicle transport independently of exogenous chemoattractant. PIP5K1C-90 polarization was required for polarized RhoA activation at uropods and provided an initial directional cue for neutrophil polarization on the endothelium. Importantly, the polarization was also required for circumventing the inhibition of lamellipodium formation by RhoA so that neutrophils could form leading edges required for transendothelial migration. Because integrins are not known to regulate neutrophil polarization, our study revealed a previously underappreciated role of integrin signaling in neutrophil regulation.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Professor Experimental Physics VI - Biologial Physics > Professor Experimental Physics VI - Biologial Physics - Univ.-Prof. Dr. Holger Kreß Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Professor Experimental Physics VI - Biologial Physics
Result of work at the UBT:	No
DDC Subjects:	500 Science > 530 Physics 500 Science > 570 Life sciences, biology
Date Deposited:	26 Feb 2021 09:16
Last Modified:	13 Sep 2022 13:31
URI:	https://eref.uni-bayreuth.de/id/eprint/63446