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Tumor Cell Identification by Means of Raman Spectroscopy in Combination with Optical Traps and microfluidic environments

Title data

Dochow, Sebastian ; Krafft, Christoph ; Neugebauer, Ute ; Bocklitz, Thomas ; Henkel, Thomas ; Albert, Jens ; Popp, Jürgen:
Tumor Cell Identification by Means of Raman Spectroscopy in Combination with Optical Traps and microfluidic environments.
In: Lab on a Chip. Vol. 11 (2011) Issue 8 . - pp. 1484-1490.
ISSN 1473-0189
DOI: https://doi.org/10.1039/C0LC00612B

Abstract in another language

Raman spectroscopy has been recognized to be a powerful tool for label-free discrimination of cells. Sampling methods are under development to utilize the unique capabilities to identify cells in body fluids such as saliva, urine or blood. The current study applied optical traps in combination with Raman spectroscopy to acquire spectra of single cells in microfluidic glass channels. Optical traps were realized by two 1070 nm single mode fibre lasers. Microflows were controlled by a syringe pump system. A novel microfluidic glass chip was designed to inject single cells, modify the flow speed, accommodate the laser fibres and sort cells after Raman based identification. Whereas the integrated microchip setup used 514 nm for excitation of Raman spectra, a quartz capillary setup excited spectra with 785 nm laser wavelength. Classification models were trained using linear discriminant analysis to differentiate erythrocytes, leukocytes, acute myeloid leukaemia cells (OCI-AML3), and breast tumour cells BT-20 and MCF-7 with accuracies that are comparable with previous Raman experiments of dried cells and fixed cells in a Petri dish. Implementation into microfluidic environments enables a high degree of automation that is required to improve the throughput of the approach for Raman activated cell sorting.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Computer Science > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz
Result of work at the UBT: No
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 19 May 2023 09:23
Last Modified: 19 May 2023 09:23
URI: https://eref.uni-bayreuth.de/id/eprint/76300