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Single cell analysis in native tissue : Quantification of the retinoid content of hepatic stellate cells

Titelangaben

Galler, Kerstin ; Requardt, Robert Pascal ; Glaser, Uwe ; Markwart, Robby ; Bocklitz, Thomas ; Bauer, Michael ; Popp, Jürgen ; Neugebauer, Ute:
Single cell analysis in native tissue : Quantification of the retinoid content of hepatic stellate cells.
In: Scientific Reports. Bd. 6 (2016) . - 24155.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep24155

Abstract

Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC’s anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive wavelength 785 nm is introduced for the assessment of the retinoid state of single HSCs in freshly isolated, unprocessed murine liver lobes. A quantitative estimation of the cellular retinoid content is derived. Implications of the retinoid content on hepatic health state are reported. The Raman-based results are integrated with histological assessments of the tissue samples. This spectroscopic approach enables single cell analysis regarding an important cellular feature in unharmed tissue.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Mathematik, Physik und Informatik > Institut für Informatik > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
Eingestellt am: 19 Mai 2023 08:49
Letzte Änderung: 19 Mai 2023 08:49
URI: https://eref.uni-bayreuth.de/id/eprint/76305