at Google ScholarTitle data
Hartmann, Katharina ; Putsche-Becker, Melanie ; Bocklitz, Thomas ; Pachmann, Katharina ; Niendorf, Axel ; Rösch, Petra ; Popp, Jürgen:
A study of Docetaxel-induced effects in MCF-7 cells by means of Raman microspectroscopy.
In: Analytical and Bioanalytical Chemistry.
Vol. 403
(2012)
.
- pp. 745-753.
ISSN 1618-2650
DOI: https://doi.org/10.1007/s00216-012-5887-9
Abstract in another language
Chemotherapies feature a low success rate of about 25%, and therefore, the choice of the most effective cytostatic drug for the individual patient and monitoring the efficiency of an ongoing chemotherapy are important steps towards personalized therapy. Thereby, an objective method able to differentiate between treated and untreated cancer cells would be essential. In this study, we provide molecular insights into Docetaxel-induced effects in MCF-7 cells, as a model system for adenocarcinoma, by means of Raman microspectroscopy combined with powerful chemometric methods. The analysis of the Raman data is divided into two steps. In the first part, the morphology of cell organelles, e.g. the cell nucleus has been visualized by analysing the Raman spectra with k-means cluster analysis and artificial neural networks and compared to the histopathologic gold standard method hematoxylin and eosin staining. This comparison showed that Raman microscopy is capable of displaying the cell morphology; however, this is in contrast to hematoxylin and eosin staining label free and can therefore be applied potentially in vivo. Because Docetaxel is a drug acting within the cell nucleus, Raman spectra originating from the cell nucleus region were further investigated in a next step. Thereby we were able to differentiate treated from untreated MCF-7 cells and to quantify the cell–drug response by utilizing linear discriminant analysis models.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Computer Science > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 530 Physics |
| Date Deposited: | 17 May 2023 12:21 |
| Last Modified: | 17 May 2023 12:21 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/76321 |