Multimodal nonlinear microscopy of head and neck carcinoma : toward surgery assisting frozen section analysis

Titelangaben

Heuke, Sandro ; Chernavskaia, Olga ; Bocklitz, Thomas ; Legesse, Fisseha Bekele ; Meyer, Tobias ; Akimov, Denis ; Dirsch, Olaf ; Ernst, Günther ; von Eggeling, Ferdinand ; Petersen, Iver ; Guntinas-Lichius, Orlando ; Schmitt, Michael ; Popp, Jürgen:
Multimodal nonlinear microscopy of head and neck carcinoma : toward surgery assisting frozen section analysis.
In: Head & Neck. Bd. 38 (2016) Heft 10 . - S. 1545-1552.
ISSN 1097-0347
DOI: https://doi.org/10.1002/hed.24477

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Abstract

Background
Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs.
Methods
Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second-harmonic generation (SHG) microscopy.
Results
An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a p value of 1.8·10−10. A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4-class model.
Conclusion
Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload.