at Google ScholarTitle data
März, Anne ; Mönch, Bettina ; Walter, Angela ; Bocklitz, Thomas ; Schumacher, Wilm ; Rösch, Petra ; Kiehntopf, Michael ; Popp, Jürgen:
The multifunctional application of microfluidic lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS) within the field of bioanalytics.
In:
Clinical and Biomedical Spectroscopy and Imaging II. -
Bellingham, Wash.
: SPIE
,
2011
. - 8087-07
. - (Proceedings of SPIE
; 8087
)
ISBN 978-0-8194-8684-4
DOI: https://doi.org/10.1364/ECBO.2011.808707
Abstract in another language
This contribution will present a variety of applications of lab-on-a-chip surface enhanced Raman spectroscopy in the field of bioanalytic. Beside the quantification and online monitoring of drugs and pharmaceuticals, determination of enzyme activity and discrimination of bacteria are successfully carried out utilizing LOC-SERS. The online-monitoring of drugs using SERS in a microfluidic device is demonstrated for nicotine. The enzyme activity of thiopurine methyltransferase (TPMT) in lysed red blood cells is determined by SERS in a lab-on-a-chip device. To analyse the activity of TPMT the metabolism of 6-mercaptopurine to 6-methylmercaptopurine is investigated. The discrimination of bacteria on strain level is carried out with different E. coli strains. For the investigations, the bacteria are busted by ultra sonic to achieve a high information output. This sample preparation provides the possibility to detect SERS spectra containing information of the bacterial cell walls as well as of the cytoplasm. This contribution demonstrates the great potential of LOC-SERS in the field of bioanalytics.
Further data
| Item Type: | Article in a book |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Computer Science > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 530 Physics |
| Date Deposited: | 16 May 2023 10:00 |
| Last Modified: | 16 May 2023 10:00 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/76342 |