Towards detection and identification of circulating tumour cells using Raman spectroscopy

Title data

Neugebauer, U. ; Bocklitz, Thomas ; Clement, J. H. ; Krafft, C. ; Popp, J.:
Towards detection and identification of circulating tumour cells using Raman spectroscopy.
In: Analyst. Vol. 135 (2010) Issue 12 . - pp. 3178-3182.
ISSN 0003-2654
DOI: https://doi.org/10.1039/c0an00608d

Official URL:

Abstract in another language

Body fluids are easily accessible and contain valuable indices for medical diagnosis. Fascinating tools are tumour cells circulating in the peripheral blood of cancer patients. As these cells are extremely rare, they constitute a challenge for clinical diagnostics. In this contribution we present the Raman spectroscopic-based identification of different single cells in suspension that are found in peripheral blood of cancer patients including healthy cells like leukocytes and erythrocytes, and tumour cells like leukaemic cells and cells originating from solid tumours. Leukocytes and erythrocytes were isolated from the peripheral blood of healthy donors while myeloid leukaemia cells (OCI-AML3) and breast carcinoma derived cells (MCF-7, BT-20) were obtained from cell cultures. A laser emitting 785 nm light was used for optical trapping the single cells in the laser focus and to excite the Raman spectrum. Support vector machines were applied to develop a supervised classification model with spectra of 1210 cells originating from three different donors and three independent cultivation batches. Distinguishing tumour cells from healthy cells was achieved with a sensitivity of >99.7 and a specificity of >99.5. In addition, the correct cell types were predicted with an accuracy of approximately 92%.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Computer Science > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz
Result of work at the UBT:	No
DDC Subjects:	500 Science > 530 Physics
Date Deposited:	15 May 2023 13:39
Last Modified:	15 May 2023 13:39
URI:	https://eref.uni-bayreuth.de/id/eprint/76347