at Google ScholarTitle data
Silge, Anja ; Bocklitz, Thomas ; Ossig, Rainer ; Schnekenburger, Jürgen ; Rösch, Petra ; Popp, Jürgen:
The interaction of an amino-modified ZrO₂ nanomaterial with macrophages : An in situ investigation by Raman microspectroscopy.
In: Analytical and Bioanalytical Chemistry.
Vol. 408
(2016)
.
- pp. 5935-5943.
ISSN 1618-2650
DOI: https://doi.org/10.1007/s00216-016-9710-x
Abstract in another language
Metal oxide nanoparticles (NP) are applied in the fields of biomedicine, pharmaceutics, and in consumer products as textiles, cosmetics, paints, or fuels. In this context, the functionalization of the NP surface is a common method to modify and modulate the product performance. A chemical surface modification of NP such as an amino-functionalization can be used to achieve a positively charged and hydrophobic surface. Surface functionalization is known to affect the interaction of nanomaterials (NM) with cellular macromolecules and the responses of tissues or cells, like the uptake of particles by phagocytic cells. Therefore, it is important to assess the possible risk of those modified NP for human health and environment. By applying Raman microspectroscopy, we verified in situ the interaction of amino-modified ZrO2 NP with cultivated macrophages. The results demonstrated strong adhesion properties of the NP to the cell membrane and internalization into the cells. The intracellular localization of the NP was visualized via Raman depth scans of single cells. After the cells were treated with sodium azide (NaN3) and 2-deoxy-glucose to inhibit the phagocytic activity, NP were still detected inside cells to comparable percentages. The observed tendency of amino-modified ZrO2 NP to interact with the cultivated macrophages may influence membrane integrity and cellular functions of alveolar macrophages in the respiratory system.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Computer Science > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry |
| Date Deposited: | 15 May 2023 09:01 |
| Last Modified: | 15 May 2023 09:01 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/76372 |