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Hausig, Franziska ; Sobotta, Fabian H. ; Richter, Friederike ; Harz, Dominic O. ; Traeger, Anja ; Brendel, Johannes C.:
Correlation between Protonation of Tailor-Made Polypiperazines and Endosomal Escape for Cytosolic Protein Delivery.
In: ACS Applied Materials & Interfaces.
Bd. 13
(2021)
Heft 30
.
- S. 35233-35247.
ISSN 1944-8252
DOI: https://doi.org/10.1021/acsami.1c00829
Abstract
Responsive polymers, which become protonated at decreasing pH, are considered a milestone in the development of synthetic cell entry vectors. Exact correlations between their properties and their ability to escape the endosome, however, often remain elusive due to hydrophobic interactions or limitations in the design of water-soluble materials with suitable basicity. Here, we present a series of well-defined, hydrophilic polypiperazines, where systematic variation of the amino moiety facilitates an unprecedented fine-tuning of the basicity or pKa value within the physiologically relevant range (pH 6–7.4). Coincubation of HEK 293T cells with various probes, including small fluorophores or functioning proteins, revealed a rapid increase of endosomal release for polymers with pKa values above 6.5 or 7 in serum-free or serum-containing media, respectively. Similarly, cytotoxic effects became severe at increased pKa values (>7). Although the window for effective transport appears narrow, the discovered correlations offer a principal guideline for the design of effective polymers for endosomal escape.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | membrane leakage; calcein release; endosomolytic polymers; pH-responsive polymers; basicity |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Makromolekulare Chemie I |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Eingestellt am: | 21 Feb 2024 07:25 |
| Letzte Änderung: | 21 Feb 2024 07:25 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/88588 |