Literature by the same author
plus at Google Scholar

Bibliografische Daten exportieren
 

Self‐Degrading Multifunctional PEG‐Based Hydrogels : Tailormade Substrates for Cell Culture

Title data

Kowalczuk, Kathrin ; Dasgupta, Anindita ; Páez Larios, Francisco ; Ulrich, Hans F. ; Wegner, Valentin ; Brendel, Johannes C. ; Eggeling, Christian ; Mosig, Alexander S. ; Schacher, Felix H.:
Self‐Degrading Multifunctional PEG‐Based Hydrogels : Tailormade Substrates for Cell Culture.
In: Macromolecular Bioscience. (2024) . - 2300383.
ISSN 1616-5195
DOI: https://doi.org/10.1002/mabi.202300383

Abstract in another language

The use of PEG-based hydrogels as cell culture matrix to mimic the natural extracellular matrix (ECM) has been realized using a range of well-defined, tunable, and dynamic scaffolds, although they require cell adhesion ligands such as RGDS-peptide (Arg-Gly-Asp-Ser) to promote cell adhesion. Herein the synthesis of ionic and degradable hydrogels is demonstrated for cell culture by crosslinking [PEG-SH]4 with the zwitterionic crosslinker N,N-bis(acryloxyethyl)-N-methyl-N-(3-sulfopropyl) ammonium betaine (BMSAB) and the cationic crosslinker N,N-bis(acryloxyethyl)-N,N-dimethyl-1-ammonium iodide (BDMAI). Depending on the amount of ionic crosslinker used in gel formation, the hydrogels show tunable gelation time and stiffness. At the same time, the ionic groups act as catalysts for hydrolytic degradation, thereby allowing to define a stability window. The latter could be tailored in a straightforward manner by introducing the non-degradable crosslinker tri(ethylene glycol) divinyl ether. In addition, both ionic crosslinkers favor cell attachment in comparison to the pristine PEG hydrogels. The degradation is examined by swelling behavior, rheology, and fluorescence correlation spectroscopy indicating degradation kinetics depending on diffusion of incorporated fluorescent molecules.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry I
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry I > Chair Macromolecular Chemistry I - Univ.-Prof. Dr. Johannes C. Brendel
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 20 Feb 2024 09:27
Last Modified: 02 May 2024 07:39
URI: https://eref.uni-bayreuth.de/id/eprint/88594