Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Title data

Speckner, Konstantin ; Rehfeldt, Florian ; Weiss, Matthias:
Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin.
In: Physical Review E. Vol. 110 (18 July 2024) Issue 1 . - 014406.
ISSN 2470-0053
DOI: https://doi.org/10.1103/PhysRevE.110.014406

Official URL:

Abstract in another language

The interior of cellular nuclei, the nucleoplasm, is a crowded fluid that is pervaded by protein-decorated DNA polymers, the chromatin. Due to the complex architecture of chromatin and a multitude of associated nonequilibrium processes, e.g., DNA repair, the nucleoplasm can be expected to feature nontrivial material properties and hence anomalous transport phenomena. Here, we have used single-particle tracking on nuclear actin rods to probe such transport phenomena. Our analysis reveals that short actin rods in the nucleus show an intermittent, antipersistent subdiffusion with clear signatures of fractional Brownian motion. Moreover, the diffusive motion is heterogeneous with clear signatures of an intermittent switching of trajectories between at least two different mobilities, most likely due to transient associations with chromatin. In line with this interpretation, hyperosmotic stress is seen to stall the motion of nuclear actin rods, whereas hypo-osmotic conditions yield a reptationlike motion. Our data highlights the heterogeneity of transport in the nucleoplasm that needs to be taken into account for an understanding of nucleoplasmic organization and the mechanobiology of nuclei.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter > Chair Experimental Physics I - Physics of Living Matter - Univ.-Prof. Dr. Matthias Weiss Faculties Faculties > Faculty of Mathematics, Physics und Computer Science Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Chair Experimental Physics I - Physics of Living Matter
Result of work at the UBT:	Yes
DDC Subjects:	500 Science > 530 Physics 500 Science > 570 Life sciences, biology
Date Deposited:	22 Jul 2024 07:10
Last Modified:	23 Jul 2024 06:22
URI:	https://eref.uni-bayreuth.de/id/eprint/90016