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Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy

Title data

Mayer, Moritz ; Schug, Christina ; Geimer, Stefan ; Klecker, Till ; Westermann, Benedikt:
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy.
In: Microbial Cell. Vol. 11 (2024) Issue 1 . - pp. 378-386.
ISSN 2311-2638
DOI: https://doi.org/10.15698/mic2024.11.840

Official URL: Volltext

Project information

Project title:
Project's official title
Project's id
FOR2092
239484859
Open Access Publizieren
No information

Project financing: Deutsche Forschungsgemeinschaft
Elitenetzwerk Bayern

Abstract in another language

Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM). Preparation of yeast cells for TEM can be quite challenging and time-consuming. Here, we describe an optimized protocol for conventional fixation of yeast cells with potassium permanganate combined with cell wall permeabilization with sodium metaperiodate and embedding in Epon. We have replaced time-consuming incubation steps by short treatments with microwaves and developed a microwave-assisted permanganate fixation and Epon embedding protocol that reduces the time required for sample preparation to one working day. We expect that these protocols will be useful for routine analysis of membrane ultrastructure in yeast.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Saccharomyces cerevisiae; microwave fixation; mitochondria; organelle architecture; potassium permanganate fixation; transmission electron microscopy;
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professor Cell Biology > Professor Cell Biology - Univ.-Prof. Dr. Benedikt Westermann
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 28 Jan 2025 07:56
Last Modified: 02 Apr 2025 07:48
URI: https://eref.uni-bayreuth.de/id/eprint/91715