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Dannah, Nora S. ; Nabeel-Shah, Syed ; Kurat, Christoph F. ; Sabatinos, Sarah A. ; Fillingham, Jeffrey:
Functional Analysis of Hif1 Histone Chaperone in Saccharomyces cerevisiae.
In: G3 : Genes | Genomes | Genetics.
Bd. 8
(2018)
Heft 6
.
- S. 1993-2006.
ISSN 2160-1836
DOI: https://doi.org/10.1534/g3.118.200229
Abstract
The Hif1 protein in the yeast is an evolutionarily conserved H3/H4-specific chaperone and a subunit of the nuclear Hat1 complex that catalyzes the acetylation of newly synthesized histone H4. Hif1, as well as its human homolog NASP, has been implicated in an array of chromatin-related processes including histone H3/H4 transport, chromatin assembly and DNA repair. In this study, we elucidate the functional aspects of Hif1 Initially we establish the wide distribution of Hif1 homologs with an evolutionarily conserved pattern of four tetratricopeptide repeats (TPR) motifs throughout the major fungal lineages and beyond. Subsequently, through targeted mutational analysis, we demonstrate that the acidic region that interrupts the TPR2 is essential for Hif1 physical interactions with the Hat1/Hat2-complex, Asf1, and with histones H3/H4. Furthermore, we provide evidence for the involvement of Hif1 in regulation of histone metabolism by showing that cells lacking are both sensitive to histone H3 over expression, as well as synthetic lethal with a deletion of histone mRNA regulator We also show that a basic patch present at the extreme C-terminus of Hif1 is essential for its proper nuclear localization. Finally, we describe a physical interaction with a transcriptional regulatory protein Spt2, possibly linking Hif1 and the Hat1 complex to transcription-associated chromatin reassembly. Taken together, our results provide novel mechanistic insights into Hif1 functions and establish it as an important protein in chromatin-associated processes.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Asf1; H3/H4 chaperone; Hat1; Hif1p; NASP; SHNi TPR; chromatin |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften |
| Eingestellt am: | 06 Aug 2026 07:59 |
| Letzte Änderung: | 06 Aug 2026 07:59 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/99207 |