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Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates

Title data

Kurat, Christoph F. ; Yeeles, Joseph T. P. ; Patel, Harshil ; Early, Anne ; Diffley, John F. X.:
Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.
In: Molecular Cell. Vol. 65 (2017) Issue 1 . - pp. 117-130.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2016.11.016

Abstract in another language

The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted this process in vitro. We show that chromatin enforces DNA replication origin specificity by preventing non-specific MCM helicase loading. Helicase activation occurs efficiently in the context of chromatin, but subsequent replisome progression requires the histone chaperone FACT (facilitates chromatin transcription). The FACT-associated Nhp6 protein, the nucleosome remodelers INO80 or ISW1A, and the lysine acetyltransferases Gcn5 and Esa1 each contribute separately to maximum DNA synthesis rates. Chromatin promotes the regular priming of lagging-strand DNA synthesis by facilitating DNA polymerase α function at replication forks. Finally, nucleosomes disrupted during replication are efficiently re-assembled into regular arrays on nascent DNA. Our work defines the minimum requirements for chromatin replication in vitro and shows how multiple chromatin factors might modulate replication fork rates in vivo.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: DNA replication; biochemistry; chromatin
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics II > Chair Genetics II - Univ.-Prof. Dr. Christoph Kurat
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics II
Result of work at the UBT: No
DDC Subjects: 500 Science > 500 Natural sciences
Date Deposited: 06 Aug 2026 07:58
Last Modified: 01 Oct 2026 08:32
URI: https://eref.uni-bayreuth.de/id/eprint/99208