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Isolation and characterization of Arabidopsis halleri and Thlaspi caerulescens phytochelatin synthases

Title data

Meyer, Claire-Lise ; Peisker, Daniel ; Courbot, Mikael ; Cracium, Adrian R. ; Cazalé, Anne-Claire ; Desgain, Denis ; Schat, Henk ; Clemens, Stephan ; Verbruggen, Nathalie:
Isolation and characterization of Arabidopsis halleri and Thlaspi caerulescens phytochelatin synthases.
In: Planta. Vol. 234 (2011) Issue 1 . - pp. 83-95.
ISSN 0032-0935
DOI: https://doi.org/10.1007/s00425-011-1378-z

Abstract in another language

The synthesis of phytochelatins (PC) represents a major metal and metalloid detoxification mechanism in various species. PC most likely play a role in the distribution and accumulation of Cd and possibly other metals. However, to date, no studies have investigated the phytochelatin synthase (PCS) genes and their expression in the Cd-hyperaccumulating species. We used functional screens in two yeast species to identify genes expressed by two Cd hyperaccumulators (Arabidopsis halleri and Thlaspi caerulescens) and involved in cellular Cd tolerance. As a result of these screens, PCS genes were identified for both species. PCS1 was in each case the dominating cDNA isolated. The deduced sequences of AhPCS1 and TcPCS1 are very similar to AtPCS1 and their identity is particularly high in the proposed catalytic N-terminal domain. We also identified in A. halleri and T. caerulescens orthologues of AtPCS2 that encode functional PCS. As compared to A. halleri and A. thaliana, T. caerulescens showed the lowest PCS expression. Furthermore, concentrations of PC in Cd-treated roots were the highest in A. thaliana, intermediate in A. halleri and the lowest in T. caerulescens. This mirrors the known capacity of these species to translocate Cd to the shoot, with T. caerulescens being the best translocator. Very low or undetectable concentrations of PC were measured in A. halleri and T. caerulescens shoots, contrary to A. thaliana. These results suggest that extremely efficient alternative Cd sequestration pathways in leaves of Cd hyperaccumulators prevent activation of PC synthase by Cd2+ ions.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER93112
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Apr 2015 15:42
Last Modified: 07 Mar 2016 14:32
URI: https://eref.uni-bayreuth.de/id/eprint/11699