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Upgrading a microplate reader for photobiology and all-optical experiments

Title data

Richter, Florian ; Scheib, Ulrike ; Mehlhorn, Jennifer ; Schubert, Roman ; Wietek, Jonas ; Gernetzki, Oliver ; Hegemann, Peter ; Mathes, Tilo ; Möglich, Andreas:
Upgrading a microplate reader for photobiology and all-optical experiments.
In: Photochemical & Photobiological Sciences. Vol. 14 (February 2015) Issue 2 . - pp. 270-279.
ISSN 1474-9092
DOI: https://doi.org/10.1039/c4pp00361f

Project information

Project financing: DFG
Einstein-Stiftung

Abstract in another language

Automation can vastly reduce the cost of experimental labor and thus facilitate high experimental throughput, but little off-the-shelf hardware for the automation of illumination experiments is commercially available. Here, we use inexpensive open-source electronics to add programmable illumination capabilities to a multimode microplate reader. We deploy this setup to characterize light-triggered phenomena in three different sensory photoreceptors. First, we study the photoactivation of Arabidopsis thaliana phytochrome B by light of different wavelengths. Second, we investigate the dark-state recovery kinetics of the Synechocystis sp. blue-light sensor Slr1694 at multiple temperatures and imidazole concentrations; while the kinetics of the W91F mutant of Slr1694 are strongly accelerated by imidazole, the wild-type protein is hardly affected. Third, we determine the light response of the Beggiatoa sp. photoactivatable adenylate cyclase bPAC in Chinese hamster ovary cells. bPAC is activated by blue light in dose-dependent manner with a half-maximal intensity of 0.58 mW cm(-2); intracellular cAMP spikes generated upon bPAC activation decay with a half time of about 5 minutes after light switch-off. Taken together, we present a setup which is easily assembled and which thus offers a facile approach to conducting illumination experiments at high throughput, reproducibility and fidelity.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 25373866
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 15 May 2015 09:07
Last Modified: 01 Jun 2017 06:48
URI: https://eref.uni-bayreuth.de/id/eprint/13506