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Autocatalytic folding of the folding catalyst FKBP12

Title data

Scholz, Christian ; Zarnt, Toralf ; Kern, Gunther ; Lang, Karl ; Burtscher, Helmut ; Fischer, Gunter ; Schmid, Franz X.:
Autocatalytic folding of the folding catalyst FKBP12.
In: The Journal of Biological Chemistry. Vol. 271 (31 May 1996) Issue 22 . - pp. 12703-12707.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.271.22.12703

Abstract in another language

Prolyl isomerases are folding enzymes and thus have the potential to catalyze their own folding. We show here that the folding of cytosolic FKBP12 (FK 506 binding protein) is an autocatalytic process both for the mature protein and for a fusion protein with an amino-terminal extension of 16 residues. Native FKBP contains seven trans-prolyl peptide bonds, and the cis-to-trans isomerizations of some or all of them constitute the slow, rate-limiting events in folding. The rate of an autocatalytic reaction increases with reactant concentration, because the product catalyzes its own formation. Accordingly, the folding of the fusion protein was more than 10-fold accelerated when the protein concentration was increased from 0.05 microM to 10 microM. At high concentrations of both forms of FKBP12 autocatalysis was very efficient, and the observed folding rate seemed to approach the rate of the fast direct folding reaction of the protein molecules with the correct (all trans) peptidyl-prolyl bond conformation.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 8662669
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 18 May 2015 07:04
Last Modified: 18 May 2015 07:04
URI: https://eref.uni-bayreuth.de/id/eprint/13515