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From dusk till dawn : one-plasmid systems for light-regulated gene expression

Title data

Ohlendorf, Robert ; Vidavski, Roee R. ; Eldar, Avigdor ; Moffat, Keith ; Möglich, Andreas:
From dusk till dawn : one-plasmid systems for light-regulated gene expression.
In: Journal of Molecular Biology. Vol. 416 (2 March 2012) Issue 4 . - pp. 534-542.
ISSN 0022-2836
DOI: https://doi.org/10.1016/j.jmb.2012.01.001

Project information

Project financing: Alexander von Humboldt-Stiftung
National Instituts of Health

Abstract in another language

Signaling photoreceptors mediate diverse organismal adaptations in response to light. As light-gated protein switches, signaling photoreceptors provide the basis for optogenetics, a term that refers to the control of organismal physiology and behavior by light. We establish as novel optogenetic tools the plasmids pDusk and pDawn, which employ blue-light photoreceptors to confer light-repressed or light-induced gene expression in Escherichia coli with up to 460-fold induction upon illumination. Key features of these systems are low background activity, high dynamic range, spatial control on the 20-μm scale, independence from exogenous factors, and ease of use. In optogenetic experiments, pDusk and pDawn can be used to specifically perturb individual nodes of signaling networks and interrogate their role. On the preparative scale, pDawn can induce by light the production of recombinant proteins and thus represents a cost-effective and readily automated alternative to conventional induction systems.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 22245580
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 19 May 2015 08:23
Last Modified: 19 May 2015 08:23
URI: https://eref.uni-bayreuth.de/id/eprint/13599