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Semisynthesis of a homogeneous glycoprotein enzyme : ribonuclease C: part 2

Title data

Piontek, Christian ; Varón Silva, Daniel ; Heinlein, Christian ; Pöhner, Claudia ; Mezzato, Stefano ; Ring, Petra ; Martin, Andreas ; Schmid, Franz X. ; Unverzagt, Carlo:
Semisynthesis of a homogeneous glycoprotein enzyme : ribonuclease C: part 2.
In: Angewandte Chemie International Edition. Vol. 48 (2009) Issue 11 . - pp. 1941-1945.
ISSN 1521-3773
DOI: https://doi.org/10.1002/anie.200804735

Abstract in another language

Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 19180621
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Bioorganic Chemistry > Chair Bioorganic Chemistry - Univ.-Prof. Dr. Carlo Unverzagt
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Bioorganic Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 02 Jun 2015 09:00
Last Modified: 02 Jun 2015 09:00
URI: https://eref.uni-bayreuth.de/id/eprint/14574