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Piontek, Christian ; Varón Silva, Daniel ; Heinlein, Christian ; Pöhner, Claudia ; Mezzato, Stefano ; Ring, Petra ; Martin, Andreas ; Schmid, Franz X. ; Unverzagt, Carlo:
Semisynthesis of a homogeneous glycoprotein enzyme : ribonuclease C: part 2.
In: Angewandte Chemie International Edition.
Bd. 48
(2009)
Heft 11
.
- S. 1941-1945.
ISSN 1521-3773
DOI: https://doi.org/10.1002/anie.200804735
Abstract
Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.