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Phytochelatin synthesis is essential for the detoxification of excess Zn and contributes significantly to the accumulation of Zinc

Title data

Tennstedt, Pierre ; Peisker, Daniel ; Böttcher, Christoph ; Trampczynska, Aleksandra ; Clemens, Stephan:
Phytochelatin synthesis is essential for the detoxification of excess Zn and contributes significantly to the accumulation of Zinc.
In: Plant Physiology. Vol. 149 (2009) Issue 2 . - pp. 938-948.
ISSN 1532-2548
DOI: https://doi.org/10.1104/pp.108.127472

Abstract in another language

The synthesis of phytochelatins (PCs) is essential for the detoxification of non-essential metals and metalloids such as cadmium and arsenic in plants and a variety of other organisms. No direct evidence for a role of PCs in essential metal homeostasis has been reported to date. Prompted by observations in S. pombe and S. cerevisiae indicating a contribution of phytochelatin synthase (PCS) expression to Zn2+ sequestration we investigated a known phytochelatin-deficient Arabidopsis thaliana mutant, cad1-3, and a newly isolated second strong allele, cad1-6, with respect to Zn homeostasis. We found that in a medium with low cation content PC-deficient mutants show pronounced Zn2+ hypersensitivity. This phenotype is of comparable strength to the well-documented Cd2+ hypersensitivity of cad1 mutants. PC deficiency also results in significant reduction in root Zn accumulation. In order to be able to sensitively measure phytochelatin accumulation we established an assay using capillary liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (CapLC-ESI-QTOF) of derivatized extracts. Plants grown under control conditions consistently showed PC2 accumulation. Analysis of plants treated with same-effect concentrations revealed that Zn2+-elicited PC2 accumulation in roots reached about 30 % of the level of Cd2+-elicited PC2 accumulation. We conclude from these data, that PC formation is essential for Zn2+ tolerance and provides driving force for the accumulation of Zn. This function might also help explain the mysterious occurrence of PCS genes throughout the plant kingdom and in a wide range of other organisms.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER66430
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 06 Jul 2015 10:25
Last Modified: 06 Jul 2015 10:25
URI: https://eref.uni-bayreuth.de/id/eprint/15912