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Boch, Annegret ; Trampczynska, Aleksandra ; Simm, Claudia ; Taudte, Nadine ; Krämer, Ute ; Clemens, Stephan:
Loss of Zhf and the tightly regulated zinc-uptake system SpZrt1 in Schizosaccharomyces pombe reveals the delicacy of cellular zinc balance.
In: FEMS Yeast Research.
Bd. 8
(2008)
Heft 6
.
- S. 883-896.
ISSN 1567-1364
DOI: https://doi.org/10.1111/j.1567-1364.2008.00414.x
Abstract
Zn is an essential micronutrient yet can be toxic when present in excess. Zn acquisition and distribution are dependent on tightly controlled transport of Zn2+ ions. Schizosaccharomyces pombe represents a second eukaryotic model to study cellular metal homeostasis. In several ways its micronutrient metabolism is fundamentally different from Saccharomyces cerevisiae. We identified the first Zn2+ uptake system in S. pombe and named it SpZrt1. Knock-out strains for all three ZIP transporters in fission yeast were constructed. Only zrt1Δ cells were unable to grow at low Zn2+ and showed reduced 65Zn2+ uptake. Elemental profiles revealed a strong decrease in Zn accumulation. Cd2+ ions inhibited uptake but not Fe2+ or Mn2+. Both mRNA abundance and protein amount are tightly regulated. Zrt1 activity is rapidly shut down upon transfer of Zn-deficient cells to Zn-replete conditions. In cells lacking Zhf, a transporter mediating ER storage of Zn, this response is about 100fold more sensitive. Thus, removal of excess of Zn from the cytosol is largely Zhf-dependent. Moreover, cells deficient for both transporters are no longer able to adjust to changing external Zn2+ concentrations. Optimal growth is restricted to a narrow range of Zn2+ concentrations illustrating the fine balance between micronutrient deficiency and toxicity.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER61522 |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Pflanzenphysiologie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Pflanzenphysiologie > Lehrstuhl Pflanzenphysiologie - Univ.-Prof. Dr. Stephan Clemens Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 06 Jul 2015 10:25 |
| Letzte Änderung: | 13 Jul 2015 06:16 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/15928 |