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Loss of Zhf and the tightly regulated zinc-uptake system SpZrt1 in Schizosaccharomyces pombe reveals the delicacy of cellular zinc balance

Title data

Boch, Annegret ; Trampczynska, Aleksandra ; Simm, Claudia ; Taudte, Nadine ; Krämer, Ute ; Clemens, Stephan:
Loss of Zhf and the tightly regulated zinc-uptake system SpZrt1 in Schizosaccharomyces pombe reveals the delicacy of cellular zinc balance.
In: FEMS Yeast Research. Vol. 8 (2008) Issue 6 . - pp. 883-896.
ISSN 1567-1364
DOI: https://doi.org/10.1111/j.1567-1364.2008.00414.x

Abstract in another language

Zn is an essential micronutrient yet can be toxic when present in excess. Zn acquisition and distribution are dependent on tightly controlled transport of Zn2+ ions. Schizosaccharomyces pombe represents a second eukaryotic model to study cellular metal homeostasis. In several ways its micronutrient metabolism is fundamentally different from Saccharomyces cerevisiae. We identified the first Zn2+ uptake system in S. pombe and named it SpZrt1. Knock-out strains for all three ZIP transporters in fission yeast were constructed. Only zrt1Δ cells were unable to grow at low Zn2+ and showed reduced 65Zn2+ uptake. Elemental profiles revealed a strong decrease in Zn accumulation. Cd2+ ions inhibited uptake but not Fe2+ or Mn2+. Both mRNA abundance and protein amount are tightly regulated. Zrt1 activity is rapidly shut down upon transfer of Zn-deficient cells to Zn-replete conditions. In cells lacking Zhf, a transporter mediating ER storage of Zn, this response is about 100fold more sensitive. Thus, removal of excess of Zn from the cytosol is largely Zhf-dependent. Moreover, cells deficient for both transporters are no longer able to adjust to changing external Zn2+ concentrations. Optimal growth is restricted to a narrow range of Zn2+ concentrations illustrating the fine balance between micronutrient deficiency and toxicity.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER61522
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 06 Jul 2015 10:25
Last Modified: 13 Jul 2015 06:16
URI: https://eref.uni-bayreuth.de/id/eprint/15928