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Metabolome Analysis of Biosynthetic Mutants Reveals a Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis

Title data

Böttcher, Christoph ; von Roepenack-Lahaye, Edda ; Schmidt, Jürgen ; Schmotz, Constanze ; Neumann, Steffen ; Scheel, Dierk ; Clemens, Stephan:
Metabolome Analysis of Biosynthetic Mutants Reveals a Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis.
In: Plant Physiology. Vol. 147 (2008) Issue 4 . - pp. 2107-2120.
ISSN 1532-2548
DOI: https://doi.org/10.1104/pp.108.117754

Abstract in another language

Metabolomics is facing a major challenge: the lack of knowledge about metabolites present in a given biological system. Thus, large-scale discovery of metabolites is considered an essential step towards a better understanding of plant metabolism. We show here that the application of a metabolomics approach generating structural information for the analysis of A. thaliana mutants allows efficient cataloging of metabolites. 56 % of the features that showed significant differences in abundance between seeds of wild type, tt4 and tt5 mutant plants could be annotated. Seventy five compounds were structurally characterized, 21 of which could be identified. About 40 compounds had not been known from A. thaliana before. Also, the high resolution analysis revealed an unanticipated expansion of metabolic conversions upstream of biosynthetic blocks. Deficiency in chalcone synthase results in the increased seed-specific biosynthesis of a range of phenolic choline esters. Similarly, a lack of chalcone isomerase activity leads to the accumulation of various naringenin chalcone derivatives. Furthermore, our data provide insight into the connection between p-coumaroyl-CoA-dependent pathways. Lack of flavonoid biosynthesis results in elevated synthesis not only of p-coumarate derived choline esters but also of sinapate-derived metabolites. However, sinapoylcholine is not the only accumulating end product. Instead, we observed specific and sophisticated changes in the complex pattern of sinapate-derivatives.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER61514
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 06 Jul 2015 10:25
Last Modified: 06 Jul 2015 10:25
URI: https://eref.uni-bayreuth.de/id/eprint/15934