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Sucrose unloading in the hypocotyl of the Ricinus communis L. seedling measured by 13C-nuclear magnetic resonance spectroscopy in vivo

Title data

Kalusche, Bernhard ; Verscht, Jutta ; Gebauer, Gerhard ; Komor, Ewald ; Haase, Axel:
Sucrose unloading in the hypocotyl of the Ricinus communis L. seedling measured by 13C-nuclear magnetic resonance spectroscopy in vivo.
In: Planta. Vol. 208 (1999) Issue 3 . - pp. 358-364.
ISSN 0032-0935
DOI: https://doi.org/10.1007/s004250050570

Abstract in another language

The unloading of sucrose in the apical part of the hypocotyl of Ricinus communis L. seedlings was measured by 13C-nuclear magnetic resonance (NMR) spectroscopy. The cotyledons of the seedling were immersed in 5 mM Mes buffer containing 100 mM 13C-labeled sucrose. At intervals of 70-90 min, (C-NMR)-13C spectra with broadband decoupling and nuclear Overhauser enhancement were acquired in vivo. The spectra showed growing 13C-resonances of the labeled positions in the sucrose molecule reaching steady-state labeling within 7-8 h. The specific 13C labeling of sucrose in the G(1)-position changed from 0.38 in the supplied sucrose solution to 0.16 in the sucrose extracted from the hypocotyl piece at the end of the experiment (13 h). Labeling of starch (and other insolubles) in the hypocotyl piece was ca. 0.10. It is proposed that the decreased specific labeling of unloaded sucrose is mostly due to the separate local pools of sucrose in the cortex and pith parenchyma, respectively, and less to continuous starch degradation and conversion to sucrose. The report gives an example of the application of 13C-NMR spectroscopy in assimilate allocation studies.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER22430
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Ecology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 24 Sep 2015 09:38
Last Modified: 24 Sep 2015 09:38
URI: https://eref.uni-bayreuth.de/id/eprint/19713