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Nitrate content and nitrate reductase activity in Rumex obtusifolius L. I. Differences in organs and diurnal changes

Title data

Gebauer, Gerhard ; Melzer, Arnulf ; Rehder, H.:
Nitrate content and nitrate reductase activity in Rumex obtusifolius L. I. Differences in organs and diurnal changes.
In: Oecologia. Vol. 63 (1984) Issue 1 . - pp. 136-142.
ISSN 1432-1939
DOI: https://doi.org/10.1007/BF00379795

Abstract in another language

With Rumex obtusifolius L., the influence of some environmental conditions on nitrate uptake and reduction were investigated. Nitrate concentrations of plant material were determined by HPLC, the activity of nitrate reductase by an in vivo test. As optimal incubation medium, a buffer containing 0.04 M KNO3; 0.25 M KH2PO4; 1.5% propanol (v/v); pH 8.0 was found. Vacuum infiltration caused an increase of enzyme activity of up to 40%.High nitrate concentrations were found in roots and leaf petioles. Nitrate reductase activity of these organs, however, was low. On the other hand, the highest nitrate reductase activity was observed in leaf laminae, which contained lowest nitrate concentrations.In leaves, nitrate content and nitrate reductase activity exhibited inverse diurnal fluctuations. During darkness, decreasing activities of the enzyme were followed by increasing nitrate concentrations, while during light the contrary was true. In petioles diurnal fluctuations in nitrate content were observed, too. No significant correlations with illumination, however, could be found.Our results prove that Rumex obtusifolius is characterized by an intensive nitrate turnover. Theoretically, internal nitrate content of the plant would be exhausted within a few hours, if a supply via the roots would be excluded.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER25888
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Ecology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 12 Nov 2015 07:45
Last Modified: 12 Nov 2015 07:45
URI: https://eref.uni-bayreuth.de/id/eprint/22515