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Shear rheology of a cell monolayer

Titelangaben

Fernandez, Pablo ; Heymann, Lutz ; Ott, Albrecht ; Aksel, Nuri ; Pullarkat, Pramod A.:
Shear rheology of a cell monolayer.
In: New Journal of Physics. Bd. 9 (2007) Heft 11 . - S. 419.
ISSN 1367-2630
DOI: https://doi.org/10.1088/1367-2630/9/11/419

Volltext

Link zum Volltext (externe URL): Volltext

Abstract

We report a systematic investigation of the mechanical properties of fibroblast cells using a novel cell monolayer rheology (CMR) technique. The new technique provides quantitative rheological parameters averaged over ?106 cells making the experiments highly reproducible. Using this method, we are able to explore a broad range of cell responses not accessible using other present day techniques. We perform harmonic oscillation experiments and step shear or step stress experiments to reveal different viscoelastic regimes. The evolution of the live cells under externally imposed cyclic loading and unloading is also studied. Remarkably, the initially nonlinear response becomes linear at long timescales as well as at large amplitudes. Within the explored rates, nonlinear behaviour is only revealed by the effect of a nonzero average stress on the response to small, fast deformations. When the cell cytoskeletal crosslinks are made permanent using a fixing agent, the large amplitude linear response disappears and the cells exhibit a stress stiffening response instead. This result shows that the dynamic nature of the cross-links and/or filaments is responsible for the linear stress-strain response seen under large deformations. We rule out the involvement of myosin motors in this using the inhibitor drug blebbistatin. These experiments provide a broad framework for understanding the mechanical responses of the cortical actin cytoskeleton of fibroblasts to different imposed mechanical stimuli.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Biorheologie; Zellen
Institutionen der Universität: Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Lehrstuhl Experimentalphysik I - Physik lebender Materie
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Technische Mechanik und Strömungsmechanik
Fakultäten
Fakultäten > Fakultät für Mathematik, Physik und Informatik
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut
Fakultäten > Fakultät für Ingenieurwissenschaften
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 18 Nov 2015 10:06
Letzte Änderung: 18 Nov 2015 10:06
URI: https://eref.uni-bayreuth.de/id/eprint/22604