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Cell Cycle Regulation of the Chicken hsp90α Expression

Title data

Jérôme, Valérie ; Vourc'h, Claire ; Baulieu, Etienne-Emile ; Catelli, Maria-Grazia:
Cell Cycle Regulation of the Chicken hsp90α Expression.
In: Experimental Cell Research. Vol. 205 (March 1993) Issue 1 . - pp. 44-51.
ISSN 1090-2422
DOI: https://doi.org/10.1006/excr.1993.1056

Official URL: Volltext

Abstract in another language

The heat-shock protein of 90 kDa (hsp90), constitutively expressed in most cells, is up-regulated by thermal stress and by developmental and mitogenic stimuli. When the serum-starved chicken hepatoma cell line DU249 is stimulated by serum, insulin, or growth factors acting via tyrosine kinase receptors, a transient induced expression of the hsp90α gene takes place at both the messenger RNA and the protein synthesis level. This response is protein synthesis dependent and DNA synthesis independent. The maximum level of hsp90α mRNA accumulation always preceeds the maximum level of thymidine incorporation, suggesting a possible link between cell cycle and hsp90α regulation (Jérôme, V., J. Léger, J. Devin, E. E. Baulieu, and M. G. Catelli. Growth Factors 4:317-327, 1991). Herein, we examine the subcellular distribution of hsp90 and the cell cycle-dependent regulation of the hsp90α mRNA level. We show that, in contrast to hsp70, the 35 S metabolically-labeled hsp90, which accumulates in the cytosoluble fraction 6 to 8 h after serum treatment, is not preferentially translocated to the nuclear compartment, although a small fraction is always present in the nucleus. We also demonstrated that serum- or insulin-induced accumulation of hsp90α mRNA results from an activation of gene transcription and that hsp90α promoter activity, which is low in quiescent DU249 cells, is induced ~fivefold after serum stimulation. Finally, in cell culture synchronized by nocodazole or aphidicholin, hsp90α mRNA accumulation seems an event specific to G₁/S transition.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
Date Deposited: 26 Feb 2016 13:00
Last Modified: 04 Mar 2016 11:16
URI: https://eref.uni-bayreuth.de/id/eprint/31090