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Cell Cycle Regulation of the Chicken hsp90α Expression


Jérôme, Valérie ; Vourc'h, Claire ; Baulieu, Etienne-Emile ; Catelli, Maria-Grazia:
Cell Cycle Regulation of the Chicken hsp90α Expression.
In: Experimental Cell Research. Bd. 205 (März 1993) Heft 1 . - S. 44-51.
ISSN 1090-2422
DOI: https://doi.org/10.1006/excr.1993.1056


Link zum Volltext (externe URL): Volltext


The heat-shock protein of 90 kDa (hsp90), constitutively expressed in most cells, is up-regulated by thermal stress and by developmental and mitogenic stimuli. When the serum-starved chicken hepatoma cell line DU249 is stimulated by serum, insulin, or growth factors acting via tyrosine kinase receptors, a transient induced expression of the hsp90α gene takes place at both the messenger RNA and the protein synthesis level. This response is protein synthesis dependent and DNA synthesis independent. The maximum level of hsp90α mRNA accumulation always preceeds the maximum level of thymidine incorporation, suggesting a possible link between cell cycle and hsp90α regulation (Jérôme, V., J. Léger, J. Devin, E. E. Baulieu, and M. G. Catelli. Growth Factors 4:317-327, 1991). Herein, we examine the subcellular distribution of hsp90 and the cell cycle-dependent regulation of the hsp90α mRNA level. We show that, in contrast to hsp70, the 35 S metabolically-labeled hsp90, which accumulates in the cytosoluble fraction 6 to 8 h after serum treatment, is not preferentially translocated to the nuclear compartment, although a small fraction is always present in the nucleus. We also demonstrated that serum- or insulin-induced accumulation of hsp90α mRNA results from an activation of gene transcription and that hsp90α promoter activity, which is low in quiescent DU249 cells, is induced ~fivefold after serum stimulation. Finally, in cell culture synchronized by nocodazole or aphidicholin, hsp90α mRNA accumulation seems an event specific to G₁/S transition.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 26 Feb 2016 13:00
Letzte Änderung: 04 Mär 2016 11:16
URI: https://eref.uni-bayreuth.de/id/eprint/31090