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Distribution of phosphatase activity and various bacterial phyla in the rhizosphere of Hordeum vulgare L. depending on P availability

Title data

Spohn, Marie ; Treichel, Nicole S. ; Cormann, Michael ; Schloter, Michael ; Fischer, Doreen:
Distribution of phosphatase activity and various bacterial phyla in the rhizosphere of Hordeum vulgare L. depending on P availability.
In: Soil Biology & Biochemistry. Vol. 89 (2015) . - pp. 44-51.
ISSN 0038-0717
DOI: https://doi.org/10.1016/j.soilbio.2015.06.018

Abstract in another language

Despite the importance of the rhizosphere for nutrient turnover, little is known about the spatial patterns of organic phosphorus mineralization by plants and by microorganisms in the rhizosphere.Therefore, the distribution of acid and alkaline phosphatase activity and the abundance of bacteria belonging to various bacterial phyla were investigated in the rhizosphere of barley (Hordeum vulgare L.) as dependent on the availability of inorganic P. For this purpose, we conducted a greenhouse experiment with barley growing in inclined boxes that can be opened to the bottom side (rhizoboxes), and appliedsoil zymography and fluorescence-in situ-hybridization (FISH). Acid phosphatase activity was strongly associated with the root and was highest at the root tips. Due to P fertilization, acid phosphatase activity decreased in the bulk soil, and less strongly in the rhizosphere. Alkaline phosphatase activity, i.e., microbial phosphatase activity was high throughout the soil in the control treatment and was reduced due to inorganic P fertilization especially in the rhizosphere and less strongly in the bulk soil. P-fertilization slightly increased the total number of bacteria in the rhizosphere. Moreover, P-fertilization decreased the abundance of Firmicutes and increased the abundances of Beta- and Gamma-Proteobacteria. The totalnumber of bacterial cells was significantly higher at the root surface than at the root tip and at a distance of 30 µm from the root surface. Our results show that alkaline phosphatase activity decreased more strongly in the rhizosphere than in the bulk soil due to P fertilization, which might be because of greater C deficiency in the bulk soil compared to the rhizosphere. Furthermore, the results indicate a spatial separation between hotspots of acid phosphatase activity and hotspots of bacteria in the rhizosphere of H. vulgare. Taken together, our study shows that bacteria and phosphatase activity were very heterogeneouslydistributed in soil, and that the effects of P fertilization on phosphatase activity differedstrongly between bulk soil and rhizosphere as well as between various zones of the rhizosphere.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER126613
Institutions of the University: Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Soil Ecology
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 04 Aug 2016 07:29
Last Modified: 04 Aug 2016 07:29
URI: https://eref.uni-bayreuth.de/id/eprint/33819