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Redundant ERF-VII transcription factors bind an evolutionarily conserved cis-motif to regulate hypoxia-responsive gene expression in Arabidopsis

Title data

Gasch, Philipp ; Fundinger, Moritz ; Müller, Jana T. ; Lee, Travis ; Bailey-Serres, Julia ; Mustroph, Angelika:
Redundant ERF-VII transcription factors bind an evolutionarily conserved cis-motif to regulate hypoxia-responsive gene expression in Arabidopsis.
In: The Plant Cell. Vol. 28 (2016) Issue 1 . - pp. 160-180.
ISSN 1532-298X
DOI: https://doi.org/10.1105/tpc.15.00866

Abstract in another language

The response of Arabidopsis thaliana to low-oxygen stress (hypoxia), such as during shoot submergence or root waterlogging, includes increasing the levels of ∼50 hypoxia-responsive gene transcripts, many of which encode enzymes associated with anaerobic metabolism. Upregulation of over half of these mRNAs involves stabilization of five group VII ethylene response factor (ERF-VII) transcription factors, which are routinely degraded via the N-end rule pathway of proteolysis in an oxygen- and nitric oxide-dependent manner. Despite their importance, neither the quantitative contribution of individual ERF-VIIs nor the cis-regulatory elements they govern are well understood. Here, using single- and double-null mutants, the constitutively synthesized ERF-VIIs RELATED TO APETALA2.2 (RAP2.2) and RAP2.12 are shown to act redundantly as principle activators of hypoxia-responsive genes; constitutively expressed RAP2.3 contributes to this redundancy, whereas the hypoxia-induced HYPOXIA RESPONSIVE ERF1 (HRE1) and HRE2 play minor roles. An evolutionarily conserved 12-bp cis-regulatory motif that binds to and is sufficient for activation by RAP2.2 and RAP2.12 is identified through a comparative phylogenetic motif search, promoter dissection, yeast one-hybrid assays, and chromatin immunopurification. This motif, designated the hypoxia-responsive promoter element, is enriched in promoters of hypoxia-responsive genes in multiple species.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER133188
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Plant Genetics
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Plant Genetics > Professorship Plant Genetics- Univ.Prof. Dr. Angelika Mustroph
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 10 Aug 2016 10:21
Last Modified: 10 Aug 2016 10:21
URI: https://eref.uni-bayreuth.de/id/eprint/33881