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Smart Core-Shell Microgel Support for Acetyl Coenzyme A Synthetase : a Step Toward Efficient Synthesis of Polyketide-Based Drugs

Title data

Dubey, Nidhi Chandrama ; Tripathi, Bijay Prakash ; Stamm, Manfred ; Ionov, Leonid:
Smart Core-Shell Microgel Support for Acetyl Coenzyme A Synthetase : a Step Toward Efficient Synthesis of Polyketide-Based Drugs.
In: Biomacromolecules. Vol. 15 (14 July 2014) Issue 7 . - pp. 2776-2783.
ISSN 1526-4602
DOI: https://doi.org/10.1021/bm5006382

Abstract in another language

The flexibility in tuning the structure and charge properties of PNIPAm microgels during their synthesis makes them a suitable choice for various biological applications. Two-step free radical polymerization, a common method employed for synthesis of core–shell microgel has been well adopted to obtain cationic poly(N-isopropylacrylamide-aminoethyl methacrylate) (PNIPAm-AEMA) shell and PNIPAm core. Scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta potential, and ninhydrin assay suggests nearly monodispersed particles of cationic nature. Amino groups on the microgel provides suitable attachment point for covalent immobilization of acetyl coenzyme A synthetase (Acs) via 1-ethyl-3-(3-N,N- dimethylaminopropyl) carbodiimide (EDC) chemistry. On immobilization, 61.55% of initial activity of Acs has been retained, while Michaelis–Menten kinetics of the immobilized Acs indicates identical Km (Michaelis constant) but decrease in the Vmax (maximum substrate conversion rate) compared to free enzyme. Immobilized Acs shows an improvement in activity at wide temperature and pH range and also demonstrates good thermal, storage, and operational stability. The Acs–microgel bioconjugate has been successfully reused for four consecutive operation cycles with more than 50% initial activity.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Professur Biofabrikation > Professur Biofabrikation - Univ.-Prof. Dr. Leonid Ionov
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Professur Biofabrikation
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 21 Jun 2017 11:25
Last Modified: 21 Jun 2017 11:25
URI: https://eref.uni-bayreuth.de/id/eprint/37711